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Plasma has emerged as a convenient source for diagnostics for a variety of biological conditions, including cancer. Detection of human papillomavirus (HPV) DNA has been shown to be of potential use for screening women for cervical cancer and for monitoring HPV-related disease. We used the polymerase chain reaction (PCR) with various primer sets in conjunction with Southern blot hybridization to investigate human plasma for viral DNA of HPV-16 and HPV-18. Using primers specific for HPV-16, of the 13 cervical carcinomas and 9 paraffin-embedded cervical adenocarcinomas tested, 4 (31%) had detectable HPV-16 DNA in plasma, 3 (23%) had detectable HPV-18 DNA, and 1 (8%) had both HPV-16 and HPV-18 DNA. None of 5 normal individuals had HPV-16 or HPV-18 DNA in plasma. A cervical biopsy specimen of 1 patient had detectable HPV-16 DNA in plasma, but not in biopsy tissue. A 4-fold increase in the plasma levels of HPV-16 DNA was detected over the course of a 5-week follow-up period. Plasma levels of HPV-16 DNA in 4 patients were analyzed in relation to the stage of disease at presentation. All patients had detectable HPV-16 DNA in plasma throughout the follow-up period. High levels of plasma HPV-16 DNA was detected in the 3 patients with metastases. These results demonstrate the presence of HPV-16 DNA in the plasma of patients with invasive cervical carcinoma. These results further demonstrate the
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Oxydoras reznikowi (Linnavaruola, 1998) (Monogenea: Dactylogyridae) is redescribed from the Russian Arctic population of the Siberian sturgeon (Acipenser baeri) from the Amur River, based on light and scanning electron microscopy. Based on newly obtained material, the haptoral sclerites and dorsal-ventral anchors are described. The previously reported ventral and subterminal anchors are revised and redescribed. The new data on the haptoral sclerites include the pseudosegmental borders of the branches, ventral anchors and ventral tube. New data on the dorsal-ventral anchors include the shape of the ventral bars.Art Basel Miami Beach ā about the exhibition and this yearās edition
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